Learning Objectives ATP

Learning Objectivesz Review the basic steps of ATP production (i.e., cellular respiration, lactic acid fermentation).z Identify the molecular steps involved in muscle contraction.z State the importance of ATP generation to muscle contraction.z Understand the relationship between rigor mortis and muscle contraction.The StorySteve Knox is a hard-working research biochemist. He has been married to his wife Jackie for overeight years. The project he is working on is a continuation of a project he began in graduate schoolexamining metabolic processes in the mitochondria of Xenopus laevis frogs. His technician Leslie isalso very devoted to the project and often works late hours on the weekends with Steve.Late one Saturday evening, Steve and Leslie are alone in the lab trying to finish up an experiment. As asurprise, Jackie has decided to bring Steve and Leslie a late-night snack since both of them have misseddinner. Around 11:30 p.m., Jackie enters the lab with drinks and brownies. The lights are on but no oneseems to be around.”I wonder where they are,” Jackie muses to herself. “Steve? Leslie? I brought you a surprise!”Turning a corner into the back of the lab, Jackie sees Steve and Leslie involved in a passionate kiss nearthe fume hood. Shocked, Jackie drops the food onto the floor, making a clatter. Looking equallyshocked, Steve and Leslie immediately disentangle themselves and start sputtering excuses andstraightening their clothes.”Hi, honey, uhhhh … what are you doing here?” mutters Steve.Jackie is enraged. She lunges toward Steve, grabbing his shoulders and shaking him. “What are youdoing? Don’t you love me any more? I HATE YOU! I HATE YOU!”Steve suddenly loses his balance and begins to fall. On his way down, he hits his head on the corner ofthe lab bench, falling unconscious. Jackie screams.Leslie, meanwhile, has been watching in horror. Seeing Steve on the floor, she fears for her own safety.Jackie might attack her next! Reacting quickly, she grabs a flask of a clear solution sitting on thecounter in the hood and tosses the liquid into Jackie’s face, and then runs out of the lab.Early Monday morning, a colleague discovers Steve and Jackie’s dead bodies and calls 911. Thecoroner later releases information about the estimated time of death for the two based on the extent ofrigor mortis. Steve, whose body was still warm and barely stiff, is estimated to have died approximatelyfive hours before being discovered. Jackie, whose body was completely cold and stiff, is estimated tohave died approximately 30 hours before being discovered.byClaudia Bode, Biology Department, University of KansasAllison Jablonski, Biology Department, Lynchburg CollegeHomework Questions1. Construct a time-line of the events from Saturday evening through Monday morning as describedin the story.2. Cellular Respiration Questions:a. Which part of cellular respiration uses 2 ATP and produces 4 ATP per glucose molecule?Where does this set of reactions occur in the cell?b. Which part of cellular respiration produces the most ATP? How does it work?c. Which part of cellular respiration produces the most NADH? How many?d. What molecules are needed to produce ATP by oxidative phosphorylation?3. In a living organism, what happens to ATP production when there is no oxygen available(anaerobic conditions)?In-Class Questions4. What are the characteristics of a dead body?5. Consider the processes involved in muscle contraction. Summarize in a few sentences therelationship between ATP and muscle contraction.6. What is rigor mortis? Why does rigor mortis develop? What are the different stages of rigor mortis.

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